Set after the events of the film My Little Pony: A New Generation (2021), the series follows five ponies on their adventures and battles across Equestria. The ponies are joined later on by Misty Brightdawn, a shy unicorn and former associate of the series' antagonist, the villainous alicorn Opaline Arcana. The series is a collaboration between Entertainment One and Atomic Cartoons.
Sunny Starscout, Izzy Moonbow, Hitch Trailblazer, the sisters Pipp Petals and Zipp Storm, and newcomer Misty Brightdawn live in Maretime Bay. Sunny's home, the Crystal Brighthouse, now holds the Unity Crystals, special magical objects created by Twilight long ago to save the ponies of Equestria from the evil fire alicorn Opaline Arcana.