The series is set after the events of the film My Little Pony: A New Generation (2021) The series follows five ponies on their adventures across Equestria. The 44-minute special episode Make Your Mark premiered as a teaser on May 26, 2022, followed by eight further episodes on September 26.
Many generations after Twilight Sparkle became the ruler of Equestria, Sunny Starscout, Izzy Moonbow and Hitch Trailblazer live in Maretime Bay. Sunny's home, the Crystal Brighthouse, now holds the Unity Crystals, special magical objects created by Twilight long ago. The three pony kinds have been reunited, but still face some obstacles in living together and dealing with their newfound magic.