The series originally aired 125 episodes from May 5, 2006, to November 6, 2016, on the Disney Channel's preschool block, Playhouse Disney. It was succeeded by Mickey Mouse Roadster Racers (later renamed Mickey Mouse Mixed-Up Adventures), which ran from 2017 to 2021, and Mickey Mouse Funhouse, which started in 2021. On August 18, 2023, a revival was revealed to be in production, under the working title Mickey Mouse Clubhouse+, and is set to be released in August 2025.
Mickey, Minnie, Donald, Daisy, Goofy, and Pluto interact with the viewer to stimulate problem solving during a self contained story. Mickey invites the viewers to join him at the Mousekedoer, a giant Mickey-head-shaped computer whose main function is to distribute the day's tools to Mickey. The show features two original songs performed by American alternative rock band They Might Be Giants, including the opening theme song.